Publications

Peer Reviewed Journal Articles

Cover of Cells 2022 Vol. 11 Issue 21
  1. Agarwal, D, Dash, N., Mazo, K., Chopra, M., Garcia, Maria PA., Patel, P., Wong, R., Jia, C., Do, H., Cheng, J., Chiang, C., Jurlina, S., Perry, M., Rho, J., Broyer, R., Lee, C., Weinreb, RN., Gavrilovici, C., Oesch, N., Welsbie, DS., Wahlin, KJ. Human retinal ganglion cell neurons generated by synchronous BMP inhibition and transcription factor mediated reprogramming. NPJ Regenerative Medicine. 2023. https://www.nature.com/articles/s41536-023-00327-x
  2. Agarwal, D.; Do, H.; Mazo, K.W.; Chopra, M.; Wahlin, K.J. Restoring Vision and Rebuilding the Retina by Müller Glial Cell Reprogramming. Stem Cell Research 2023, 66, 103006, https://doi.org/10.1016/j.scr.2022.103006.
  3. Jurlina, S.L.; Jones, M.K.; Agarwal, D.; De La Toba, D.V.; Kambli, N.; Su, F.; Martin, H.M.; Anderson, R.; Wong, R.M.; Seid, J.; Attaluri, S.V.; Chow, M.; Wahlin, K.J. A Tet-Inducible CRISPR Platform for High-Fidelity Editing of Human Pluripotent Stem Cells. Genes 2022, 13, 2363. https://doi.org/10.3390/genes13122363
  4. Agarwal, D.; Kuhns, R.; Dimitriou, C.N.; Barlow, E.; Wahlin, K.J.; Enke, R.A. Bulk RNA Sequencing Analysis of Developing Human Induced Pluripotent Cell-Derived Retinal Organoids. Sci Data 2022, 9, 759, https://doi.org/10.1038/s41597-022-01853-x.
  5. Jones, M.K.; Agarwal, D.; Mazo, K.W.; Chopra, M.; Jurlina, S.L.; Dash, N.; Xu, Q.; Ogata, A.R.; Chow, M.; Hill, A.D.; Kambli, N.K.; Xu, G.; Sasik, R.; Birmingham, A.; Fisch, K.M.; Weinreb, R.N.; Enke, R.A.; Skowronska-Krawczyk, D.; Wahlin, K.J. Chromatin Accessibility and Transcriptional Differences in Human Stem Cell-Derived Early-Stage Retinal Organoids. Cells 2022, 11, 3412. https://doi.org/10.3390/cells11213412
  6. Maiti, G., de Barros, M. R. M., Hu, N., Dolgalev, I., Roshan, M., Foster, J. W., Tsirigos, A., Wahlin, K. J., & Chakravarti, S. (2022). Single-cell RNA-seq of human cornea organoids identifies cell fates of a developing immature cornea. PNAS Nexus, pgac246. https://doi.org/10.1093/pnasnexus/pgac246
  7. Gage, E., Agarwal, D., Chenault, C., Washington-Brown, Szvetecz, Jahan, N., Zack, DJ., Enke, R., Wahlin, KJ. Temporal and isoform-specific expression of CTBP2 is evolutionarily conserved between the developing chick and human retina. Frontiers in Molecular Neuroscience. 2021.
  8. Wahlin KJ, Cheng, J., Jurlina, S., Jones, M., Ogata, A., Kibria, N., Ray, S., Eldred, K., Kim, C., Heng, J., Phillips, J., Johnston, R., Gamm, D., Berlinicke, C., Zack, D. CRISPR generated SIX6 and POU4F2 reporters allow identification of brain and optic transcriptional differences in human PSC-derived organoids. Frontiers in Cell and Developmental Biology. Nov 16, 2021
  9. Sripathi SR, Hu MW, Mertz JL, Liu MM, Wan J, Maruotti J, Wahlin KJ, Berlinicke CA, Qian J, Zack DJ. Epithelial to mesenchymal transition (EMT) of human stem cell-derived retinal pigment epithelium shares commonalities with malignancy-associated EMT: a proteomic analysis. Molecular & Cellular Proteomics. 2021
  10. Sripathi SR, Hu MW, Liu MM, Wan J, Cheng J, Duan Y, Mertz JL, Wahlin KJ, Maruotti J, Berlinicke CA, Qian J, Zack DJ. Transcriptome Landscape of Epithelial to Mesenchymal Transition of Human Stem Cell-Derived RPE. Invest Ophthalmol Vis Sci. 2021 Apr 1;62(4):1. doi: 10.1167/iovs.62.4.1. PubMed PMID: 33792620; PubMed Central PMCID: PMC8024778.
  11. Biswas P, Borooah S, Matsui H, Voronchikhina M, Zhou J, Zawaydeh Q, Raghavendra PB, Ferreyra H, Riazuddin SA, Wahlin KJ, Frazer KA, Ayyagari R. Detection and validation of novel mutations in MERTK in a simplex case of retinal degeneration using WGS and hiPSC-RPEs model. Hum Mutat. 2021 Feb;42(2):189-199. doi: 10.1002/humu.24146. Epub 2020 Dec 13. PubMed PMID: 33252167; PubMed Central PMCID: PMC7878419.
  12. Patel, AK., Broyer, RM., Lee, CD., Lu, T., Louie, MJ., La Torre, A., Al-Ali, H., Vu, MT., Mitchell, KL., Wahlin, KJ., Berlinicke, CA., Jaskula-Ranga, V., Hu, Y., Duan, X., Vilar, S., Bixby, JL., Weinreb, RN., Lemmon, VP., Zack, DJ., Welsbie, DS. Inhibition of GCK-IV kinases dissociates cell death and axon regeneration in CNS neurons. Patel AK, Broyer RM, Lee CD, Lu T, Louie MJ, La Torre A, Al-Ali H, Vu MT, Mitchell KL, Wahlin KJ, Berlinicke CA, Jaskula-Ranga V, Hu Y, Duan X, Vilar S, Bixby JL, Weinreb RN, Lemmon VP, Zack DJ, Welsbie DS. Proc Natl Acad Sci U S A. 2020 PubMed PMID: 33318207; PMCID:PMC7777023.
  13. Huang KC, Wang ML, Chen SJ, Kuo JC, Wang WJ, Nhi Nguyen PN, Wahlin KJ, Lu JF, Tran AA, Shi M, Chien Y, Yarmishyn AA, Tsai PH, Yang TC, Jane WN, Chang CC, Peng CH, Schlaeger TM, Chiou SH. Morphological and Molecular Defects in Human Three-Dimensional Retinal Organoid Model of X-Linked Juvenile Retinoschisis. Stem Cell Reports. 2019 Nov 12;13(5):906-923. doi: 10.1016/j.stemcr.2019.09.010. Epub 2019 Oct 24. PubMed PMID: 31668851; PubMed Central PMCID: PMC6895767
  14. Mishra, B., Wilson, D.R., Sripathi, S., Suprenant, MP., Rui, Y., Wahlin, KJ, Berlinicke, CA., Green, JJ., Zack, DJ. A Combinatorial Library of Biodegradable Polyesters Enables Non-viral Gene Delivery to Post-Mitotic Human Stem Cell-Derived Polarized RPE Monolayers. Regen. Eng. Transl. Med. (2019).
  15. Eldred, KC, Hadyniak, SE., Hussey, K., Brennerman, B., Zhang, PW., Chamling, X., Sluch, V., Welsbie, D., Hattar, S., Taylor, J., Wahlin, KJ., Zack, DJ., Johnston, RJ., Thyroid hormone signaling specifies cone subtypes in human retinal organoids. Science. 2018 Oct 12;362(6411). PMID: 30309916. PMC6249681.
  16. Aleen Remez A, Onishi A, Menuchin-Lasowski, Biran L, Blackshaw S, Wahlin KJ, Zack DJ, Ashery-Padan R. Pax6 is essential for the generation of late-born retinal neurons and for inhibition of photoreceptor-fate during late stages of retinogenesis. Dev Biol. 2017 Oct 6. pii: S0012-1606(16)30701-1. PMID: 28993200.
  17. Vergara, NM., , Flores-Bellver, M., Aparicio-Domingo, S., McNally, M., Wahlin, KJ., Saxena, M., Mumm, J., Canto-Soler, MV. Enabling quantitative screening in retinal organoids: 3D automated reporter quantification technology (3D-ARQ). 2017 Sep 4. pii: dev.146290. doi: 10.1242/dev.146290.
  18. Wilson, D.., Routkevitch, D., Mosenia, A., Wahlin, KJ., Zack, DJ., Quinones-Hinojosa, A., Green, J.  A Triple-Fluorophore Labeled Nucleic Acid pH Nanosensor to Investigate Non-Viral Gene Delivery. Molecular Therapy. Molecular Therapy. 2017.
  19. Wahlin, KJ., Maruotti, J., Sripathi, SR., Ball, J., Angueyra, J., Kim, B., Greebe, R., Li, W., Jones, BA.,  Zack, DJ. Photoreceptor Outer Segments in Long-Term 3D Retinas from Human Pluripotent Stem Cells. * Scientific Reports 7, Article number: 766 (2017)
  20. Foster, J., Wahlin KJ, Adams, S., Birk, D., Zack, DJ., Shakravarkti, S. Cornea organoids from human induced pluripotent stem cells. Scientific Reports 7, Article number: 41286 (2017).
  21. Maruotti, J., Sripathi, S., Bharti, K., Fuller, J., Wahlin, K., Sluch, V., Ranganathan, V., Berlinicke, C., Kim, C., Wan, J., Qian, J., Bhutto, I., Lutty, J., Temple, S., Olenyuk, B., Zhao, L., Zack, DJ. Small molecule directed efficient generation of retinal pigment epithelium from human pluripotent stem cells. Proc Natl Acad Sci U S A. 2015. PMID:26269569
  22. Ranganathan, V., Wahlin, KJ, Maruotti, J., Zack, DJ. Expansion of the CRISPR-Cas9 genome targeting space through the use of H1 promoter-expressed guide RNAs. Nat Commun 2014 PMCID:PMC4133144
  23. Masuda, T., Wahlin, KJ., Wan, J., Hu, J., Maruotti, J., Yang, X., Iacovelli, J., Wolkow, N., Kist, R., Dunaief, J., Qian, J., Zack, DJ., Esumi, N. SOX9 Plays a Key Role in the Regulation of Visual Cycle Gene Expression in the Retinal Pigment Epithelium.  Journal of Biological Chemistry 2014. PMCID:PMC4007478
  24. Bhise, N*., Wahlin, KJ*., Zack, DJ., Green, J. Evaluating the potential of poly(beta-amino ester) nanoparticles for reprogramming human fibroblasts to induced pluripotent stem cells. Int J. Nanomedicine. 2013.PMCID:PMC3857166.
  25. Wahin, K.J., Enke, R., Fuller, JA., Kalesnykas, G., Zack DJ., Merbs, S. Epigenetics and Cell Death: DNA Hypermethylation in Programmed Retinal Cell Death. PLOS1. 2013. PMCID:PMC3823652.
  26. Maruotti J., Wahlin KJ., Gorrell, D., Zack DJ. A simple and scalable process for the differentiation of retinal pigment epithelium from human pluripotent stem cells. Stem Cells Translational Medicine. May;2(5):341-54, 2013. PMCID:PMC3667560.
  27. Zhang L*., Wahlin, KJ*., Li Y *., Masuda, T., Yang Z., Zack DJ., Esumi N. RIT2, a neuron-specific small guanosine triphosphatase, is expressed in retinal neuronal cells and its promoter is modulated by the POU4 transcription factors. Molecular Vision. Jun 17;19:1371-86 , 2013. PMCID:PMC3692409
  28. Lumayag S, Haldin , CE., Corbett N., Wahlin ,KJ., Cowan C., Turturro S., Larsen P., Kovacs B, Witmer PD., Valle D., Zack DJ., Nicholson DA., Xu. S. Inactivation of the miR-183/96/182 Cluster Results in Syndromic Retinal Degeneration. Proc Natl Acad Sci U S A. 2013 Feb 5;110(6):E507-16. PMCID:PMC3568372.
  29. Wahlin, KJ., Hackler, L., Adler, RA, Zack, DJ. Alternative Splicing of Neuroligin and its Protein Distribution in the Outer Plexiform Layer of the Chicken Retina. J Comp Neurol. 2010 Dec 15;518(24):4938-62. 2010. PMCID:PMC4065168. 
  30. McNally MM, Wahlin KJ., Canto-Soler M. Endogenous expression of ASLV viral proteins in Specific Pathogen Free chicken embryos: relevance for the developmental biology research field. BMC Developmental Biology 18, 10:106. 2010. PMCID: PMC2973938.
  31. Engler, C., Kelliher, C., Wahlin, KJ., Speck, CL., Jun AS. Comparison of non-viral methods to genetically modify and enrich populations of primary human corneal endothelial cells. Mol vision15:629-6. 2009 PMCID:PMC2664841. 
  32. Wahlin, KJ., E.F. Moreira, H. Huang, N. Yu, and R. Adler. Molecular dynamics of photoreceptor synapse formation in the developing chick retina. J Comp Neurol 506:822-837. 2008. PMID: 18076030
  33. Huang, H., Wahlin, KJ., M. McNally, N.D. Irving, and R. Adler. 2008. Developmental regulation of muscleblind-like (MBNL) gene expression in the chicken embryo retina. Dev Dyn 237:286-296. PMID:18095352.
  34. Pasterkamp,RJ.,Dai, HN., Terman, JR., WahlinKJ., Kim, B., Bregman, BS., Popovich, PG., and Kolodkin AL. MICAL flavoprotein monooxygenases: expression during neural development and following spinal cord injuries in the rat. Mol Cell Neurosci 31:52-69. 2006. PMID:16230022
  35. Wahlin,KJ., Lim, L., Grice, EA., Campochiaro, P., Zack, DJ., ,and Adler, R. A method for analysis of gene expression in isolated mouse photoreceptor and Müller cells. Mol Vis 10:366-375, 2004. PMID:15205663
  36. Mori, K., Gehlbach, P., Ando, A., WahlinKJ., Gunther,V., McVey, D., Wei, C and Campochiaro, PA. Intraocular adenoviral vector-mediated gene transfer in proliferative retinopathies. Invest Ophthalmol Vis Sci 43:1610-1615. 2002. PMID:11980881
  37. Wahlin,KJ., Adler,R., Zack, DJ., and Campochiaro., PA. 2001. Neurotrophic signaling in normal and degenerating rodent retinas. Experimental eye research 73:693-701.PMID: 11747369.
  38. Wahlin,KJ., Campochiaro,PA., Zack, DJ., and Adler, RA. Neurotrophic factors cause activation of intracellular signaling pathways in Müller cells and other cells of the inner retina, but not photoreceptors. Invest Ophthalmol Vis Sci 41:927-936. 2000. PMID:10711715
  39. Angiopoietin 2 expression in the retina: upregulation during physiologic and pathologic neovascularization. Hackett, SF., Ozaki, H., Strauss, RW., Wahlin, KJ., Suri, C., Maisonpierre, P., Yancopoulos, G., Campochiaro, PA. Journal of Cellular Physiology 184(3):275 – 284. 2000. PMID:10911358.
  40. Mathura, J R., Jafari, N., Chang, JT., Hackett, SF., Wahlin, KJ., Della, N., Okamoto, Campochiaro, PA. Bone morphogenetic proteins-2 and -4: negative growth regulators in adult retinal pigmented epithelium. Investigative Ophthalmology & Visual Science. 41(2):592-600 2000. PMID:10670493

Book Chapters

1. Barnett, BP., Wahlin, K., Krawczyk, M., Spencer, D., Welsbie, D., Afshari, N., Chao, D. Potential of Ocular Transmission of SARS-CoV-2. Vision 2020 Sep 1;4(3):40.PMID: 32883010 PMCID: PMC7559808  DOI: 10.3390/vision4030040.

2. Hackett, SF., Ozaki, H., Strauss, RW., Wahlin, KJ., Suri, C., Maisonpierre, P., Yancopoulos, G., Campochiaro, PA. Angiopoietin 2 expression in the retina: upregulation during physiologic and pathologic neovascularization. Journal of Cellular Physiology 184(3):275 – 284. 2000. PMID:10911358

Review Articles

1. Foster J, Wahlin K, Chakravarti S. A Guide to the Development of Human Cornea Organoids from Induced Pluripotent Stem Cells in Culture. 2020. 51-58p. Available from: https://doi.org/10.1007/978-1-0716-0599-8_5.

2. Wahlin, KJ., Maruotti, J., Zack, DJ. Modeling retinal dystrophies using patient-derived induced pluripotent stem cells. Advances in experimental medicine and biology. 01/2014; 801:157-64.